vsmcs (MedChemExpress)
Structured Review

Vsmcs, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bml+284/BML-284/pmc13175694-61-1-10
Average 95 stars, based on 55 article reviews
Images
1) Product Images from "E2F5 Accelerates Vascular Smooth Muscle Cells Phenotype Switching in Diabetic Atherosclerosis through Activating Wnt/β-Catenin Pathway"
Article Title: E2F5 Accelerates Vascular Smooth Muscle Cells Phenotype Switching in Diabetic Atherosclerosis through Activating Wnt/β-Catenin Pathway
Journal: Diabetes & Metabolism Journal
doi: 10.4093/dmj.2024.0588
Figure Legend Snippet: E2F transcription factor 5 (E2F5) knockdown repressed vascular smooth muscle cells (VSMCs) phenotype switching through inactivating Wnt/β-catenin pathway. (A) Western blot for detecting wnt1 and β-catenin expression in VSMCs in different groups. (B) 5-ethynyl-2´-deoxyuridine (EdU) assay was employed for assessing the proliferative ability of VSMCs (×200). (C) Flow cytometry was employed for testing VSMCs cell cycle. (D) Transwell assay was utilized for evaluating the migrative ability of VSMCs (×100). (E) Western blot for detecting α-smooth muscle actin (α-SMA), SM22, and osteopontin (OPN) expression in VSMCs. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HG, high glucose; ox-LDL, oxidized low-density lipoprotein; si-NC, negative control siRNA; BML-284, a Wnt pathway activator; DAPI, 4´,6-diamidino-2-phenylindole. a P <0.05, b P <0.05, c P <0.05.
Techniques Used: Knockdown, Western Blot, Expressing, EdU Assay, Flow Cytometry, Transwell Assay, Negative Control
Related Articles
Concentration Assay:Article Title: Triphenyltin chloride suppresses caudal fin regeneration in zebrafish by inhibiting Wnt signaling. Article Snippet: Anthropogenic organotin pollutants, particularly triphenyltin chloride (TPTC), threaten aquatic ecosystems.. However, their impact on tissue regeneration-a critical survival mechanism-remains poorly understood.. Therefore, we used zebrafish larvae as a model organism to assess the effect of TPTC on caudal fin regeneration. Molecular Weight:Article Title: Copper exposure induced developmental toxicity and cardiotoxicity in zebrafish. Article Snippet: Copper (Cu) is a global environmental pollutant that poses a serious threat to humans and ecosystems.. Cu is cardiotoxic in rodents, but the exact mechanism has not been elucidated.. In the present study, zebrafish embryos were exposed to 0, 10, 20, and 40 μg/L Cu2+ until 72-h post-fertilization (hpf) to assess cardiotoxicity of Cu. |